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Detection of Avian Influenza Viruses in Wild Waterbirds in the Rift Valley of Kenya Using Fecal Sampling

机译:野生水鸟中禽流感病毒的检测 在肯尼亚的裂谷使用粪便取样

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摘要

Highly pathogenic avian influenza virus A/H5N1 has been reported in 11 African countries. Migratory waterbirds have the potential of introducing A/H5N1 into east Africa through the Rift Valley of Kenya. We present the results of a wild bird surveillance system for A/H5N1 and other avian influenza viruses based on avian fecal sampling in Kenya. We collected 2630 fecal samples in 2008. Viral RNA was extracted from pools of 3–5 fecal samples and analyzed for presence of avian influenza virus RNA by real-time RT-PCR. Twelve (2.3%) of the 516 sample pools were positive for avian influenza virus RNA, 2 of which were subtyped as H4N6 viruses. This is the first report of avian influenza virus in wild birds in Kenya. This study demonstrates the success of this approach in detecting avian influenza virus in wild birds and represents an efficient surveillance system for avian influenza virus in regions with limited resources.
机译:在11个非洲国家中已报告出高致病性禽流感病毒A / H5N1。迁徙水鸟有可能通过肯尼亚的裂谷将A / H5N1引入东非。我们基于肯尼亚的粪便采样,介绍了针对A / H5N1和其他禽流感病毒的野生鸟类监视系统的结果。我们在2008年收集了2630份粪便样本。从3–5份粪便样本池中提取了病毒RNA,并通过实时RT-PCR分析了禽流感病毒RNA的存在。 516个样本库中有12个(2.3%)的禽流感病毒RNA阳性,其中2个亚型为H4N6病毒。这是肯尼亚野生鸟类禽流感病毒的首次报道。这项研究证明了这种方法在检测野生鸟类禽流感病毒方面的成功,并且代表了在资源有限的地区对禽流感病毒的有效监视系统。

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